質量分析イメージングによる物質の分布と機能の可視化
in situ 質量分析の前処理としてホットメルト樹脂フィルムを媒体とした顕微レーザーマイクロディセクションにより調製する手法を開発し、組織切片上の難容性分子や15kDまでのペプチド、核酸の分析もできるようになりました。この手法により疾患の新たなバイオマーカーの同定や細胞レベルの薬物動態との機能相関の網羅解析が可能となると考えています。
(4)今作っている装置
研究業績
発表論文
Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M. Calmodulin as a Key Regulator of Exosomal Signal Peptides. Cells 12(1): 158, 2023.
Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M. Signal Sequence-Dependent Orientation of Signal Peptide Fragments to Exosomes. Int J Mol Sci 23(6): 3137, 2022.
Inoue M, Sakamoto K, Suzuki A, Nakai S, Ando A, Shiraki Y, Nakahara Y, Omura M, Enomoto A, Nakase I, Sawada M, Hashimoto N. Size and surface modification of silica nanoparticles affect the severity of lung toxicity by modulating endosomal ROS generation in macrophages. Part Fibre Toxicol 18(1): 21, 2021.
Yoshioka N, Tanaka M, Ochi K, Watanabe A, Ono K, Sawada M, Ogi T, Itoh M, Ito A, Shiraki Y, Enomoto A, Ishigami M, Fujishiro M, Ogawa Y, Suganami T. The sodium-glucose cotransporter-2 inhibitor Tofogliflozin prevents the progression of nonalcoholic steatohepatitis-associated liver tumors in a novel murine model. Biomed Pharmacother 140: 111738, 2021.
Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M. Secretion of signal peptides via extracellular vesicles. Biochem Biophys Res Commun 560: 21-26, 2021.
Ji B, Ono M, Yamasaki T, Fujinaga M, Zhang MR, Seki C, Aoki I, Kito S, Sawada M, Suhara T, Sahara N, Higuchi M. Detection of Alzheimer's disease-related neuroinflammation by a PET ligand selective for glial versus vascular translocator protein. J Cereb Blood Flow Metab 41(8): 2076-2089, 2021.
Nomura M, Toyama H, Suzuki H, Yamada T, Hatano K, Wilson AA, Ito K, Sawada M. Peripheral benzodiazepine receptor/18 kDa translocator protein positron emission tomography imaging in a rat model of acute brain injury. 35(1): 8-16, 2021.
Wang L, Li M, Lin Y, Du S, Liu Z, Ju J, Suzuki H, Sawada M, Umezawa K. Inhibition of cellular inflammatory mediator production and amelioration of learning deficit in flies by deep sea Aspergillus-derived cyclopenin. J Antibiot 73(9):622-629, 2020.
Kawakubo M, Tanaka M, Ochi K, Watanabe A, Saka-Tanaka M, Kanamori Y, Yoshioka N, Yamashita S, Goto M, Itoh M, Shirakawa I, Kanai S, Suzuki H, Sawada M, Ito A, Ishigami M, Fujishiro M, Arima H, Ogawa Y, Suganami T. Dipeptidyl peptidase-4 inhibition prevents nonalcoholic steatohepatitis-associated liver fibrosis and tumor development in mice independently of its anti-diabetic effects. Sci Rep 10(1): 983, 2020.
Hirata Y, Ito Y, Takashima M, Yagyu K, Oh-hashi K, Suzuki H, Ono K, Furuta K, Sawada M. Novel Oxindole-Curcumin Hybrid Compound for Antioxidative Stress and Neuroprotection. ACS Chemical Neurosci 11(1): 76-85, 2020.
H. Suzuki, M. Sawada. Role of Microglia in Neuroinflammation. PET/CT FOR INFLAMMATORY DISEASES: BASIC SCIENCES, TYPICAL CASES, AND REVIEW. SPRINGER-VERLAG SINGAPORE PTE LTD, 31-34, 2020.
澤田 誠. 記憶力向上の秘訣 情報を引き出すトレーニングとはFamily Academy 河合塾, 52-56, 2018.